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Bidirectionality and transcriptional activity of the EWSR1 promoter region

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Author

Summary, in English

EWSR1 is involved in chimeric proteins which play crucial roles in the development of a variety of bone and soft tissue tumors. Many of the chimeric genes involving EWSR1 have been extensively studied, whereas less is known about the wild-type (wt) gene and its regulation. As the expression of the chimeric gene is driven by the EWSR1 promoter, it is of importance to study the mechanisms regulating wt EWSR1 expression. We estimated the transcriptional activity of the EWSR1 promoter through deletion fragments driving reporter gene expression. This assay identified the 100-bp region immediately downstream of the EWSR1 transcriptional start site (+1) and the downstream region from +100 to +300 as important regions for transcriptional regulation. We also found that EWSR1 and RHBDD3, a gene located directly upstream of EWSR1 that is likely to share regulatory elements with EWSR1, were co-expressed in the tissue panels, Ewing tumor biopsies and cell lines. Thus, our results show that the EWSR1 promoter functions in a bidirectional manner, thereby regulating also RHBDD3, and identifies specific regions that strongly influence promoter activity.

Publishing year

2009-03

Language

English

Pages

641-648

Publication/Series

Oncology Reports

Volume

21

Issue

3

Document type

Journal article

Publisher

Spandidos Publications

Topic

  • Cancer and Oncology
  • Medical Genetics

Keywords

  • Animals
  • Apoptosis Regulatory Proteins
  • Base Sequence
  • Bone Neoplasms
  • Calmodulin-Binding Proteins
  • Conserved Sequence
  • Gene Expression Regulation
  • Humans
  • Molecular Sequence Data
  • Neoplasm Proteins
  • Promoter Regions, Genetic
  • RNA-Binding Proteins
  • Reverse Transcriptase Polymerase Chain Reaction
  • Sarcoma, Ewing
  • Sequence Homology, Nucleic Acid
  • Transcription, Genetic
  • Transfection
  • Journal Article
  • Research Support, Non-U.S. Gov't

Status

Published

ISBN/ISSN/Other

  • ISSN: 1791-2431