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Development of an amperometric biosensor based on acetylcholine esterase covalently bound to a new support material

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Author

  • M Khayyami
  • M T Pérez Pita
  • N Peña Garcia
  • Gillis Johansson
  • Bengt Danielsson
  • Per-Olof Larsson

Summary, in English

A new type of amperometric biosensor based on immobilised acetylcholine esterase was designed and constructed. The enzyme was immobilised on a flow-through working electrode, which was prepared from reticulated vitreous carbon (RVC) or from a composite material consisting of RVC and superporous agarose. The sensor was operated in FIA mode using acetylthiocholine as a substrate. The sensor responded to inhibitors such as paraoxon—10−9 mol was detected by the sensor in a non-optimised configuration. The practical lifetime of the sensor was at least 1 month.

Publishing year

1998

Language

English

Pages

557-563

Publication/Series

Talanta

Volume

45

Issue

3

Document type

Journal article

Publisher

Elsevier

Topic

  • Composite Science and Engineering
  • Analytical Chemistry

Keywords

  • Acetylthiocholine
  • Acetylcholine esterase
  • Biosensor
  • RVC-electrode
  • Amperometric
  • Thiocholine
  • Mediator
  • Superporous agarose gel

Status

Published

ISBN/ISSN/Other

  • ISSN: 1873-3573