Comparison of spatially and temporally resolved diffuse-reflectance measurement systems for determination of biomedical optical properties

Summary, in English

Time-resolved and spatially resolved measurements of the diffuse reflectance from biological tissue are two well-established techniques for extracting the reduced scattering and absorption coefficients. We have performed a comparison study of the performance of a spatially resolved and a time-resolved instrument at wavelengths 660 and 785 nm and also of an integrating-sphere setup at 550-800 nm. The first system records the diffuse reflectance from a diode laser by means of a fiber bundle probe in contact with the sample. The time-resolved system utilizes picosecond laser pulses and a single-photon-counting detection scheme. We extracted the optical properties by calibration using known standards for the spatially resolved system, by fitting to the diffusion equation for the time-resolved system, and by using an inverse Monte Carlo model for the integrating sphere. The measurements were performed on a set of solid epoxy tissue phantoms. The results showed less than 10% difference in the evaluation of the reduced scattering coefficient among the systems for the phantoms in the range 9-20 cm(-1), and absolute differences of less than 0.05 cm(-1) for the absorption coefficient in the interval 0.05-0.30 cm(-1). (C) 2003 Optical Society of America.

• Author: Johannes Swartling; JS Dam; Stefan Andersson-Engels
• Department/s: Atomic Physics
• Publishing year: 2003
• Language: English
• Pages: 4612-4620
• Publication/Series: Applied Optics
• Volume: 42
• Issue: 22
• Full text: - 149 kB; Download statistics
• Links: http://www.opticsinfobase.org/abstract.cfm?URI=ao-42-22-4612
• Document type: Journal article
• Publisher: Optical Society of America
• Topic: Atom and Molecular Physics and Optics
• Status: Published
• ISBN/ISSN/Other: ISSN: 2155-3165